Techne Prime Elite Instrukcja Użytkownika Pobierz pdf (Strona 22)

GENERAL SOFTWARE FEATURES

INTRODUCTION

The Prime Elite software allows the user to quickly and easily create thermal cycling programs using a

simple and clear touch screen format. The software is structured into five modules which are accessed

from the Home Screen. These allow the user to:

• Run an existing program, selecting from

templates or programs saved on the unit.

• Create a new program.

• Set instrument and program defaults and

preferences. Includes calculator tools.

• View the current status of connected units.

• View and search for files stored on the unit.

TEMPLATE PROGRAMS

To help with programming, a number of program templates are installed on the unit which are

available to copy and edit or can be run directly without changes. Details of the installed program

templates are given below.

Instrument and program defaults

Parameter

2 Step

Template

3 Step

Template

PCR

Template

Heated Lid 105ºC 105ºC 105ºC

Heated lid before program On On On

Sample cooling On On On

Pause before program Off Off Off

Initial denaturation 94ºC, 05m00s 94ºC, 05m00s 94ºC, 05m00s

Hot start Off Off Off

Final extension 72ºC, 05m00s 72ºC, 05m00s 72ºC, 07m00s

Final hold 10ºC 10ºC 10ºC

Thermal cycling conditions

2 Step

Template

3 Step

Template

PCR

Template

Stage

Number of cycles 30 30 1

Step 1 94.0ºC, 00m30s 94.0ºC, 00m30s 45.0ºC, 40m00s

Step 2 60.0ºC, 01m00s 55.0ºC, 00m30s 94.0ºC, 05m00s

Step 3 72.0ºC, 00m30s

Stage

Number of cycles 40

Step 1 94.0ºC, 01m00s

Step 2 55.0ºC, 00m50s

Step 3 72.0ºC, 01m00s

1 2 ... 17 18 19 20 21 22 23 24 25 26 27 ... 68 69

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