Techne Prime Elite Instrukcja Użytkownika Strona 1

Prime Elite OPERATOR’S MANUAL Version 2.0 02/2013 Part code: ELITE/M1

8Câble de rechange S’il s’avère nécessaire de remplacer le cordon d’alimentation, utiliser un câble de 1 mm² conforme à la norme H05VV-F relié à un

9SICHERHEITS- INFORMATIONEN Lesen Sie diese Anleitung vor Verwendung des Prime Elite bitte sorgfältig durch. ACHTUNG HOHE TEMPERATUREN STELLEN E

10 Ersatzkabel Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm2 Adernquerschnitt und Europas

11 INFORMAZIONI DI SICUREZZA Leggere attentamente il presente manuale prima di usare il Prime Elite. AVVERTENZA Le ALTE TEMPERATURE SONO PERICO

12 7. Accendere lo strumento per mezzo dell'interruttore situato sulla parte anteriore dell'unità. Cavo di ricambio Qualora occorra s

13 INFORMACIÓN DE SEGURIDAD Lea atentamente este manual antes de utilizar el Prime Elite. ADVERTENCIA LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que

14 Cable de repuesto Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm2 de código armonizado H0

15 CONTACT INFORMATION For technical, sales or servicing information, contact your local Techne distributor or: UK Bibby Scientific Ltd. Beacon

16 PRIME ELITE SPECIFICATION TEMPERATURE Block temperature range 4°C or 20ºC below ambient to 100ºC Block uniformity <±0.3ºC Temperature accur

17 INTERCONNECTIVITY Connection to PC control software Yes (Satellite units only) Software updates Free of charge from www.techne.com COMMUNIC

18 INSTALLATION See also the Safety Information sections on pages 5 to 14. Front view Prime Elite Prime Elite Satellite 1. Prime

19 Installation 1. Place the unit on a suitable flat bench, ensuring that the air inlet vents on the front and rear are free from obstruction. Two

20 GENERAL SOFTWARE FEATURES INTRODUCTION The Prime Elite software allows the user to quickly and easily create thermal cycling programs using a s

21 The Ice Bucket and Ligation programs are simple temperature holds, with the former having an infinite hold at 4ºC and the latter at 15ºC. In bo

22 OLIGONUCLEOTIDE TM CALCULATOR One of the most important parameters in a PCR is the annealing temperature of the reaction. This can be determine

23 PROGRAMMING TEXT, TEMPERATURE AND TIME This section gives instructions for the basic programming of text, temperature and time whi

24 Advanced temperature entry • First ensure the number of cycles for the stage has been set then enter the required hold temperat

25 The temperature value will now appear orange in the programming screen indicating that it contains a modified function. To

26 The temperature value will now appear orange in the programming screen indicating that it contains a modified function. Note: It is

27 The temperature value will now appear orange in the programming screen indicating that it contains a modified function. TIME

1CONTENTS PAGE INTRODUCTION 3 BEFORE USE 4 Unpacking 4 SAFETY INFORMATION 5 English 5 Français 7 Deutsch 9 Italiano 11 Español 13 CONTACT

28 To increase the step hold time per cycle: • For an increase in hold time per cycle, touch the button below Increase Per Cycle to turn t

29 To decrease the step hold time per cycle: • For a decrease in hold time per cycle, touch the button below Decrease Per Cycle to turn th

30 INSTRUMENT SETTINGS AND DEFAULTS From the Home Screen, touch the Settings button to access the instrument Settings menu. • Touch any of th

31 INSTRUMENT SETTINGS Temperature Log Recording To record the temperature logs (block temperature profile) of each run, touch the button

32 Time • Touch Time in order to enter the current time using the 24 hour clock. • Use the + and – keys to set the time values. • When

33 Change Administrator Password This allows the administrator to change the default password (1234). • From the Administrator menu touch Cha

34 PROGRAM DEFAULTS The program default settings will apply to every new program created on the Prime Elite. However individual

35 • Touch More Settings for further program defaults or Back to return to the Settings menu. Final Extension Use this to add a final exten

36 OLIGO TM CALCULATOR The oligo Tm calculator can be used to estimate the Tm of the PCR primers and help in selecting an appropriate annealing

37 • To check the Tm of another primer with the same primer and salt concentrations, touch Back and enter the new primer sequence. • Touch Rese

2The run screen 48 Searching for a program not in the shortcut list 49 Running a program not on the shortcut list 51 EDITING A PROGRAM 53 Editin

38 CREATE A NEW PROGRAM From the Home Screen, touch the Create A New Program button. This will open a screen where you can build a program by addi

39 INSERT/DELETE A STAGE A stage is a section of a program that consists of a number of individual steps that can be cycled (rep

40 INSERT/DELETE A STEP Insert a new step • Touch Insert a new step to insert the next step in the current stage. • Edit the new step te

41 User User names can be entered and linked to the program to help in searching and identifying programs. • To enter a user name,

42 Keywords Keywords can be entered and linked to the program to help in searching for related programs. • To enter a keyword, touch the N

43 Passwords A password can be created to protect the program from being edited and deleted by other users. The password will be requested if a use

44 SETTINGS • Touch the Settings button to view the instrument defaults as set up in the Settings module. Any of these parameters

45 DELETE This is used to delete an entry. First touch the required segment followed by Delete. UP AND DOWN ARROWS Use these buttons to sc

46 RUN A PROGRAM From the Home Screen, touch one of the shortcuts to run one of the listed programs, or touch Run a Program to search and sele

47 Note: If the user attempts to send a program to unit while it is already running, the warning screen shown opposite will be displayed. •

3INTRODUCTION The Prime Elite is an advanced gradient thermal cycler designed to accurately and rapidly control the temperatures of samples betwee

48 THE RUN SCREEN The Run Screen will be displayed after a program has been started. It shows the instrument status and the progress of a run.

49 Viewing a running program from the Home Screen: • Touch Current status. • Touch the area for the required instrument. • Touch View.

50 Search the programs by user • Touch Search by User and this will open the list of user names stored on the unit. • Select the required use

51 RUNNING A PROGRAM NOT ON THE SHORTCUT LIST • Once the required program has been identified, touch it to highlight it. The Program Selector Sc

52 Note: If the user attempts to send a program to unit while it is already running, the warning screen shown opposite will be displayed. •

53 EDITING A PROGRAM EDITING SAVED PROGRAMS First select the required program by searching the Run a program module. • To edit a selected

54 EDITING PASSWORD PROTECTED PROGRAMS If the edited program is password protected, touching Save will open the message box shown oppo

55 CURRENT STATUS The Current Status area of the Home Screen provides status information on the Prime Elite and any connected Satellite units. On

56 VIEW FILES The View files module opens the File Manager and allows access to all the program and temperature log files. From here it is possibl

57 Search the files by user • Touch Search By User and this will open the list of user names stored on the unit. • Select the required user

4BEFORE USE Before using the Prime Elite please make sure you have read this manual carefully. If there is any doubt relating to the proper use of

58 VIEWING A PROGRAM FILE • Touch Programs And Logs to display Only Programs. • Search for the required program file and touch it to hi

59 When opening a temperature log file, the Temperature Log Monitor screen will be displayed. To view the complete temperature log, dra

60 To copy files to a USB memory stick: • Insert a USB memory stick into the port on the front of the unit. A message screen will appear confir

61 • Touch the upper button to save the file(s) to the Prime Elite unit. • Touch Cancel to return to the File Manager screen. DELETING FI

62 UPDATING THE PRIME ELITE SOFTWARE The Prime Elite software can be updated by downloading the latest version from the Techne website www.techne.

63 FAQS Q1 How do I adjust the pressure of the heated lid for my tubes or plates? A1 No adjustment is necessary. The lid automatically appl

64 TECHNICAL SUPPORT AND SERVICING If you require further technical or application assistance please contact Techne at: E-mail: technehelp@bibby-

65 ADDITIONAL INFORMATION USER MAINTENANCE Before cleaning your unit, disconnect it from the power supply. The outer case of the Prime Elite may b

66 REPLACEMENT PARTS The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne distributor: Product

67 DECLARATION OF CONFORMITY

5SAFETY INFORMATION Please read all the information in this manual before using the Prime Elite. WARNING HIGH TEMPERATURES ARE DANGEROUS: they can

6ENVIRONMENTAL CONDITIONS The Prime Elite is designed operate under the following conditions: • Indoor use • Ambient temperature range +5ºC to +4

7L'INFORMATION DE SÛRETÉ Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Prime Elite. AVERTISSEMENT Les